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KMID : 0380420110350040295
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Journal of Prventive Veterinary Medicine
2011 Volume.35 No. 4 p.295 ~ p.298
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Evaluation of the Automated TEMPO(R) technique for the enumeration of Staphylococcus aureus in various ready-to-eat food products
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Song-Kwang-Young
Chon Jung-Whan
Kim Hye-Sung
Kim Yun-Gyeong
Kim Dong-Hyeon
Sung Chang-Hyeon
Hyeon Ji-Yeon
Park Jun-Ho
Lee Jong-Ik
Seo Kun-Ho
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Abstract
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The aim of this study was to evaluate the TEMPOⓡ STA automated most probable number (MPN) system for the enumeration of Staphylococcus aureus (S. aureus) in comparison with a standard culture method. Artificially inoculated food products with S. aureus - triangle kimbap, sliced spring onion, dried filefish fillet, danpatjuk (sweet red-bean porridge with small rice dumplings)- were tested in this study. Twenty-five grams of each of food samples were added into 225 ㎖ of sterilized phosphate buffered saline in a TEMPOⓡ stomacher bag followed by stomaching for 2 min. One milliliter of the stomached sample was added to a bottle of culture medium. Cards were filled and sealed in the automated filler and then were incubated for 24 h at 37℃. After incubation, the cards were placed in the automated TEMPOⓡ reader and MPN results were generated. For comparison with a culture method, decimal dilutions were prepared from the same homogenized samples described above, transferred onto Baird Parker and Baird Parker-Rabbit Plasma Fibrinogen (BP-RPF) agar plates, and then incubated at 37℃ for 24 h. The performance of TEMPOⓡ STA method is equivalent to the culture method using Baird Parker or BP-RPF agar count plate for the enumeration of S. aureus in foods, eliminating a time-consuming and laborious process.
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KEYWORD
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Staphylococcus aureus, TEMPO(R), Plate Count Method, Food, Enumeration
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